Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor alpha 1 antibody
    See all Nicotinic Acetylcholine Receptor alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 1
  • Specificityab28489 recognises Nicotinic Acetylcholine Receptor alpha one isoform a and b (muscle isoforms).
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide derived from residues 349 - 398 of human Nicotinic Acetylcholine Receptor alpha.

    .

  • Positive control
    • Human intestine, fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.12 µg/ml. Predicted molecular weight: 54 kDa.Can be blocked with Nicotinic Acetylcholine Receptor alpha 1 peptide (ab189888). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.

Target

  • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Tissue specificityIsoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
  • Involvement in diseaseDefects in CHRNA1 are a cause of multiple pterygium syndrome lethal type (MUPSL) [MIM:253290]. Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent.
    Note=The alpha subunit is the main focus for antibody binding in myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs.
    Defects in CHRNA1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
    Defects in CHRNA1 are a cause of congenital myasthenic syndrome fast-channel type (FCCMS) [MIM:608930]. FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor subunit alpha antibody
    • ACHA_HUMAN antibody
    • AChR antibody
    • ACHRA antibody
    • CHNRA antibody
    • Cholinergic receptor nicotinic alpha polypeptide 1 antibody
    • Cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) antibody
    • Chrna1 antibody
    • Nicotinic cholinergic receptor alpha 1 antibody
    • Schizophrenia neurophysiologic defect candidate antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody images

  • Immunohistochemical analysis of Nicotinic Acetylcholine Receptor alpha expression in paraffin embedded human intestine tissue section using 4 µg/ml ab28489. Arrows indicate positively labelled epithelial cells of intestinal villus.
  • Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489) at 0.12 µg/ml + Fetal heart lysate

    Secondary
    HRP conjugated anti-Rabbit IgG HRP conjugated anti-Rabbit IgG (diluted in 1: 50,000 - 100,000)

    Predicted band size : 54 kDa

References for Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489)

ab28489 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab28489.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"