Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody (ab48943)


  • Product name
    Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody
    See all Nicotinic Acetylcholine Receptor alpha 2 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 2
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    A region within synthetic peptide: MGPSCPVFLS FTKLSLWWLL LTPAGGEEAK RPPPRAPGDP LSSPSPTALP, corresponding to N terminal amino acids 1-50 of Human Nicotinic Acetylcholine Receptor alpha 2

  • Positive control
    • HepG2 cell lysates Raji cell lysates


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by Protein G/A affinity chromatography method.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab48943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/15000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease
    Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4) [MIM:610353]. ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACHA2_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor nicotinic alpha 2 antibody
    • Chrna2 antibody
    • Neuronal acetylcholine receptor protein subunit alpha 2 antibody
    • Neuronal acetylcholine receptor subunit alpha-2 antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody images

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody (ab48943) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

References for Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody (ab48943)

ab48943 has not yet been referenced specifically in any publications.

Product Wall

Western blot
Mouse Cell lysate - whole cell (primary hepatocyte)
Gel Running Conditions
Reduced Denaturing (8 %)
Loading amount
40 µg
primary hepatocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Mr. Dongil Kim

Verified customer

Submitted Oct 20 2015

Thank you for providing further information. As we had not previously test this antibody in mouse tissue we do not know what type of banding pattern you would receive. Based on the information that I have found, the mouse version of Nicotinic Acetylcho...

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I will happily send you a positive control if that is what is needed to verify the problem. Before I do that, I do want to make sure that sending a positive control is what is needed and not a replacement antibody. Could you provide me the answers to t...

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I am sorry that you are experiencing problems using ab48943 in western blot. To better understand the problems that you are having with the antibody would it be possible for you to provide a copy of the protocol that you are using (total protein loa...

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