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Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 3
Predicted to work with:
Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Nicotinic Acetylcholine Receptor alpha 3.
This antibody gave a positive signal in Human Spinal Cord tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801)
Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (
) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
51 kDa (possible cleavage fragment).
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"