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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Purity Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Sequence similarities Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
Cellular localization Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody images
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801)
Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein ( ab29188) at 10 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) ( ab97051) at 1/10000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 57 kDa Observed band size : 57 kDa Additional bands at : 51 kDa (possible cleavage fragment). Exposure time : 8 minutes
References for Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801)
has not yet been referenced specifically in any publications.
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