This antibody gave a positive signal in Human Spinal Cord tissue lysate.
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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801)
Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa Observed band size: 57 kDa Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 8 minutes
The band observed at 51 kDa could potentially be a cleaved form of Nicotinic Acetylcholine Receptor alpha 3 due to the presence of a 31 amino acid signal peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.