Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab151596)

Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody
    See all Nicotinic Acetylcholine Receptor alpha 4 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 74-344 of Human Nicotinic Acetylcholine Receptor alpha 4 (Uniprot ID: P43681).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human DLD1 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease
    Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A4 nicotinic receptor antibody
    • Acetylcholine receptor alpha 4 neural antibody
    • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
    • ACH 4 antibody
    • ACH4 antibody
    • ACHA4_HUMAN antibody
    • AChR antibody
    • Acra 4 antibody
    • Acra4 antibody
    • Alpha4 nAChR antibody
    • BFNC antibody
    • Cholinergic receptor nicotinic alpha 4 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
    • CHRNA 4 antibody
    • CHRNA4 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • NACHR antibody
    • NACRA 4 antibody
    • NACRA4 antibody
    • Neuronal acetylcholine receptor subunit alpha-4 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
    see all

Images

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab151596) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS-PAGE.
  • Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling Nicotinic Acetylcholine Receptor alpha 4 with ab151596 at 1/100 dilution.

References

ab151596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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