Our Abpromise guarantee covers the use of ab41172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/800. Predicted molecular weight: 70 kDa.Can be blocked with Human Nicotinic Acetylcholine Receptor alpha 4 peptide (ab99319). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.|
|ELISA||Use at an assay dependent concentration.|
ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.