Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor alpha 4 antibody
    See all Nicotinic Acetylcholine Receptor alpha 4 primary antibodies
  • Description
    Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 4
  • Specificityab77356 is not expected to cross-react with the similar alpha 2 subunit.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    HVETRAHAEERLLKK

    , corresponding to internal sequence amino acids 29-43 of Human Nicotinic Acetylcholine Receptor alpha 4

  • Positive control
    • Rat Brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
    An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • Involvement in diseaseDefects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
    • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
    • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A4 nicotinic receptor antibody
      • Acetylcholine receptor alpha 4 neural antibody
      • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
      • ACH 4 antibody
      • ACH4 antibody
      • ACHA4_HUMAN antibody
      • AChR antibody
      • Acra 4 antibody
      • Acra4 antibody
      • Alpha4 nAChR antibody
      • BFNC antibody
      • Cholinergic receptor nicotinic alpha 4 antibody
      • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
      • CHRNA 4 antibody
      • CHRNA4 antibody
      • EBN 1 antibody
      • EBN antibody
      • EBN1 antibody
      • NACHR antibody
      • NACRA 4 antibody
      • NACRA4 antibody
      • Neuronal acetylcholine receptor subunit alpha-4 antibody
      • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
      see all

    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody images

    • Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356) at 0.3 µg/ml + Rat Brain lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 70 kDa
      Observed band size : 70 kDa
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356)

    ab77356 has not yet been referenced specifically in any publications.

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    The molecular weight of the peptide was not determined.

    The molecular weight of the antibody is ca 150kDa.

    Molecular weight is not necessary to calculate the dilutions of the blocking peptide. C...

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    Vielen Dank für Ihren Anruf. Auf Ihre Anfrage hin haben wir das Blockpeptid für den Acetylcholin-Rezeptor alpha 4 (ab77356) in unseren Katalog aufgenommen. Es hat die Artikelnummer ab123011 und kostet 175 EUR plus Versandgebühren. Click here (or ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"