Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor alpha 4 antibody
    See all Nicotinic Acetylcholine Receptor alpha 4 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human Nicotinic Acetylcholine Receptor alpha 4.

  • Positive control
    • This antibody gave a positive signal in the following lysates: SW480 Whole Cell; HepG2 Whole Cell; HeLa Whole Cell; Jurkat Whole Cell; Human Brain Tissue; Rat Brain Tissue; Mouse Cerebellum Tissue; Rat Cerebellum Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab88239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in diseaseDefects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A4 nicotinic receptor antibody
    • Acetylcholine receptor alpha 4 neural antibody
    • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
    • ACH 4 antibody
    • ACH4 antibody
    • ACHA4_HUMAN antibody
    • AChR antibody
    • Acra 4 antibody
    • Acra4 antibody
    • Alpha4 nAChR antibody
    • BFNC antibody
    • Cholinergic receptor nicotinic alpha 4 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
    • CHRNA 4 antibody
    • CHRNA4 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • NACHR antibody
    • NACRA 4 antibody
    • NACRA4 antibody
    • Neuronal acetylcholine receptor subunit alpha-4 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody images

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • ICC/IF image of ab88239 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88239, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239) at 1 µg/ml

    Lane 1 : Cerebellum (Mouse) Tissue Lysate
    Lane 2 : Cerebellum (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa


    Exposure time : 4 minutes

References for Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239)

ab88239 has not yet been referenced specifically in any publications.

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