Recombinant
RabMAb

Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody [EPR4563(2)] (ab124832)

Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody [EPR4563(2)]
    See all Nicotinic Acetylcholine Receptor alpha 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4563(2)] to Nicotinic Acetylcholine Receptor alpha 4
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Nicotinic Acetylcholine Receptor alpha 4.

  • Positive control
    • SH-SY5Y, A673, C6 and Neuro-2a cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22483693
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • Involvement in disease
      Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
    • Sequence similarities
      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
    • Cellular localization
      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A4 nicotinic receptor antibody
      • Acetylcholine receptor alpha 4 neural antibody
      • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
      • ACH 4 antibody
      • ACH4 antibody
      • ACHA4_HUMAN antibody
      • AChR antibody
      • Acra 4 antibody
      • Acra4 antibody
      • Alpha4 nAChR antibody
      • BFNC antibody
      • Cholinergic receptor nicotinic alpha 4 antibody
      • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
      • CHRNA 4 antibody
      • CHRNA4 antibody
      • EBN 1 antibody
      • EBN antibody
      • EBN1 antibody
      • NACHR antibody
      • NACRA 4 antibody
      • NACRA4 antibody
      • Neuronal acetylcholine receptor subunit alpha-4 antibody
      • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
      see all

    Images

    • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody [EPR4563(2)] (ab124832) at 1/10000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    References

    This product has been referenced in:
    • Chen SP  et al. Involvement of hippocampal acetylcholinergic receptors in electroacupuncture analgesia in neuropathic pain rats. Behav Brain Funct 12:13 (2016). Read more (PubMed: 27068709) »
    • Yin Y  et al. Accumulation of human full-length tau induces degradation of nicotinic acetylcholine receptor a4 via activating calpain-2. Sci Rep 6:27283 (2016). WB . Read more (PubMed: 27277673) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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