Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody (ab26099)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor alpha 5 antibody
    See all Nicotinic Acetylcholine Receptor alpha 5 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 5
  • Specificityab26099 recognises Neuronal acetylcholine receptor protein, alpha-5 chain precursor(CHRNA5).
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    A region within a synthetic peptide: LQNCSMKFGS WTYDGSQVDI ILEDQDVDKR DFFDNGEWEI VSATGSKGNR, corresponding to internal sequence amino acids 181-230 of Human Nicotinic Acetylcholine Receptor alpha 5

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab26099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

Target

Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody images

  • Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody (ab26099) at 2.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody (ab26099)

ab26099 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15µg/mL · Temperature: 22°C
Antigen retrieval step None
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jul 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"