Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody [319] (ab24644)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor alpha 7 antibody [319]
    See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies
  • Description
    Rat monoclonal [319] to Nicotinic Acetylcholine Receptor alpha 7
  • SpecificityThis antibody binds to both native and denatured alpha 7 subunits, but does not bind to alpha 8.
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Human, Monkey
  • Immunogen

    Recombinant fragment (Chicken): Bacterially-expressed large cytoplasmic domain of Nicotinic Acetylcholine Receptor alpha 7.

  • EpitopeThis antibody binds to the cytoplasmic surface of chicken alpha 7 acetylcholine receptors between amino acid 365-384.

Properties

Applications

Our Abpromise guarantee covers the use of ab24644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
IP
IHC-P
  • Application notesFlow Cyt: 1/100 (PMID 18393628).
    IHC-P: Use at an assay dependent dilution.
    IP: 1/150.
    WB: 1/500. Predicted molecular weight: 56 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
    • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
    • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 7nAChR antibody
      • a7 nicotinic acetylcholine receptor antibody
      • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
      • ACHA7_HUMAN antibody
      • AChR antibody
      • Acra7 antibody
      • alpha 7 neuronal nicotinic acetylcholine receptor antibody
      • alpha-7 nicotinic cholinergic receptor subunit antibody
      • alpha7 antibody
      • cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
      • cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
      • cholinergic receptor, nicotinic, alpha 7 antibody
      • cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
      • CHRNA7 antibody
      • CHRNA7-2 antibody
      • NACHRA7 antibody
      • neuronal acetylcholine receptor protein, alpha-7 chain antibody
      • Neuronal acetylcholine receptor subunit alpha-7 antibody
      see all

    Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody [319] images

    • ab24644 staining Nicotinic Acetylcholine Receptor alpha 7 in rat hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Sections were PFA-fixed and subjected to antigen retrieval by autoclave prior to blocking with 8% milk for 30 minutes at 37°C. The primary antibody was diluted 1/50 with DAKO antibody diluent and incubated with the sample for 18 hours at 4°C. An undiluted HRP-conjugated Goat anti-rat polyclonal was used as the secondary antibody.

      See Abreview

    References for Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody [319] (ab24644)

    This product has been referenced in:
    • el-Mas MM  et al. Estrogen dependence of the renal vasodilatory effect of nicotine in rats: role of a7 nicotinic cholinergic receptor/eNOS signaling. Life Sci 88:187-93 (2011). WB ; Rat . Read more (PubMed: 21092740) »
    • Kox M  et al. GTS-21 inhibits pro-inflammatory cytokine release independent of the Toll-like receptor stimulated via a transcriptional mechanism involving JAK2 activation. Biochem Pharmacol 78:863-72 (2009). Flow Cyt ; Human . Read more (PubMed: 19576181) »

    See all 7 Publications for this product

    Product Wall

    Thank you for your patience. I apologize for the delay, the lab was performing tests to determine the light chain types. The isotypes are as follows:
    ab24644 - IgG1, kappa
    ab24698 - IgG2a, kappa
    ab24719 - IgG1, kappa
    I hope this hel...

    Read More

    Thank you for filling in the questionnaire and sending us the image from the blots. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I appreciate the time taken to submit furth...

    Read More

    Thank you for contacting us.  The lab has not tested for cross-reactivity to the forms you mentioned. A BLAST alignment gives the following results:   Total sequence length: 502 aa   alpha 7 & beta: 33% similarity, 174 identical positions, 201 similar ...

    Read More

    Thank you for contacting Abcam regarding ab24644. I have confirmed that this antibody has only been tested for cross reactivity with alpha 8 and does not react.  We have not tested for cross reactivity with any other human acetylcholine receptors. ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (rat hippocampus)
    Specification rat hippocampus
    Fixative Paraformaldehyde
    Antigen retrieval step Other
    Permeabilization No
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 8% · Temperature: 37°C
    Username

    Dr. 明子 新郷

    Verified customer

    Submitted Nov 24 2010

    Thank you for your enquiry. I can confirm that this antibody is a RAT monoclonal. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Thank you for your email, and I apologize for the shortage of ab24644 that you received. I also looked into the mouse vs rat discrepancy and the vial was mislabeled. This antibody is indeed a rat monoclonal. I have arranged for a free of charge rep...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Nov 04 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"