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Synthetic peptide conjugated to KLH, selected from within the amino acid range 22-71 of Human Nicotinic Acetylcholine Receptor alpha 7. (Peptide available as ab101467.)
Our Abpromise guarantee covers the use of ab10096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human Nicotinic Acetylcholine Receptor alpha 7 peptide (ab101467). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
NNK (NCI) (100 mg/kg) was administered to BALB/c mice. The mice were randomized into two groups; group one received the vehicle (PBS) (n = 8) and group two received nicotine (n = 8) by i.p. injection at a dose of 1 mg/kg three times a week for 28 weeks. Lungs from the mice were fixed in 10% neutral-buffered formalin before processing into paraffin blocks. Sections were subjected to microwave ‘antigen retrieval’ for 20 minutes on 70% power, with a 1 minute cooling period after every 5 minutes, in 0.01 M sodium citrate, pH 6.0. Slides were stained either with H&E alone or with ab10096 against α7 nAChR (1∶50 dilution). For color development the slides were treated with peroxidase substrate kit from Vector labs and developed using DAB as chromogen. After a final rinse in dH2O, sections were lightly counterstained in hematoxylin, dehydrated and mounted.
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