Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab110851)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab110851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7nAChR antibody
    • a7 nicotinic acetylcholine receptor antibody
    • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
    • ACHA7_HUMAN antibody
    • AChR antibody
    • Acra7 antibody
    • alpha 7 neuronal nicotinic acetylcholine receptor antibody
    • alpha-7 nicotinic cholinergic receptor subunit antibody
    • alpha7 antibody
    • cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
    • cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
    • cholinergic receptor, nicotinic, alpha 7 antibody
    • cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
    • CHRNA7 antibody
    • CHRNA7-2 antibody
    • NACHRA7 antibody
    • neuronal acetylcholine receptor protein, alpha-7 chain antibody
    • Neuronal acetylcholine receptor subunit alpha-7 antibody
    see all

Images

  • ab110851 at 5µg/ml staining Nicotinic Acetylcholine Receptor alpha 7 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab110851 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (skin)
Specification
skin
Fixative
perfusion fixation
Permeabilization
Yes - Triton X 100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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