Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies -
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 7 aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P36544 -
Positive control
- ICC/IF: HEK 293T cells. IHC-P: Mouse brain tissues, rat adrenal gland and human kidney tissue. WB: mouse brain lysates, mouse lymph node lysates, mouse cerebrum lysates, mouse adrenal gland lysates, mouse placenta lysates, mouse lung lysates, mouse spleen lysates, mouse thymus lysates, human SH-SY5Y cell lysates, human U-87 MG cell lysates, human U251 cell lysates, rat cerebrum lysates, rat adrenal gland lysates, rat testis lysates and human HepG2 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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WB | (3) |
1/100 - 1/1000. Predicted molecular weight: 56 kDa.
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ICC/IF | (1) |
1/500.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
WB
1/100 - 1/1000. Predicted molecular weight: 56 kDa. |
ICC/IF
1/500. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1139 Human
- Entrez Gene: 11441 Mouse
- Entrez Gene: 25302 Rat
- Omim: 118511 Human
- SwissProt: P36544 Human
- SwissProt: P49582 Mouse
- SwissProt: Q05941 Rat
- Unigene: 511772 Human
see all -
Alternative names
- 7nAChR antibody
- a7 nicotinic acetylcholine receptor antibody
- acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
see all
Images
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All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/1000 dilution
Lane 1 : Rat Cerebrum lysates
Lane 2 : Mouse Placenta lysates
Lane 3 : Mouse Spleen lysates
Lane 4 : Mouse Thymus lysates
Lane 5 : Mouse Lymph node lysates
Lysates/proteins at 1/1000 dilution per lane.
Predicted band size: 56 kDaProbed with Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody, Unconjugated (ab216485) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/1000 dilution
Lane 1 : Rat Cerebrum lysates at 1/1000 dilution
Lane 2 : Mouse Cerebrum lysates at 1/1000 dilution
Lane 3 : Rat Adrenal gland lysates at 1/1000 dilution
Lane 4 : Mouse Adrenal gland lysates at 1/1000 dilution
Lane 5 : Mouse Placenta lysates at 1/1000 dilution
Lane 6 : Mouse Lung lysates at 1/1000 dilution
Lane 7 : Rat Testis lysates at 1/1000 dilution
Lane 8 : Human HepG2 cell lysates at 1/1000 dilution
Lane 9 : Human SH-SY5Y cell lysates at 1/1000 dilution
Predicted band size: 56 kDaProbed with Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody, Unconjugated (ab216485) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/1000 dilution + Mouse lymph node lysates at 1/1000 dilution
Predicted band size: 56 kDaMouse lymph node lysates probed with Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody, Unconjugated (AB216485) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/1000 dilution
Lane 1 : Human SH-SY5Y Cell Lysates
Lane 2 : Human U-87 MG Cell Lysates
Lane 3 : Human U251 Cell Lysates
Lysates/proteins at 1/1000 dilution per lane.
Predicted band size: 56 kDaProbed with Anti-Nicotinic Acetylcholine Receptor alpha 7 polyclonal Antibody, unconjugated (AB216485) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Immunohistochemical analysis of paraformaldehyde-fixed and paraffin embedded rat adrenal gland labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.
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Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/300 dilution + mouse brain lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 56 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by Cy3 conjugated secondary Goat Anti-Rabbit IgG. DAPI was used to stain the cell nuclei.
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Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse brain tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.
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Immunofluorescent analysis of HEK293 cells labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/500 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated. DAPI staining of nuclei is shown too.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab216485 has been referenced in 13 publications.
- Zhao J et al. Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress. Redox Biol 59:102594 (2023). PubMed: 36603528
- Chen Y et al. Alpha-7 nicotinic acetylcholine receptor agonist alleviates psoriasis-like inflammation through inhibition of the STAT3 and NF-κB signaling pathway. Cell Death Discov 8:141 (2022). PubMed: 35351863
- Zhou Y et al. Electroacupuncture Ameliorates Tibial Fracture-Induced Cognitive Dysfunction by Elevating α7nAChR Expression and Suppressing Mast Cell Degranulation in the Hippocampus of Rats. Evid Based Complement Alternat Med 2022:3182220 (2022). PubMed: 35463074
- Liang F et al. Tobacco carcinogen induces tryptophan metabolism and immune suppression via induction of indoleamine 2,3-dioxygenase 1. Signal Transduct Target Ther 7:311 (2022). PubMed: 36068203
- Yang Y et al. Single-Cell Transcriptional Profiling Reveals Low-Level Tragus Stimulation Improves Sepsis-Induced Myocardial Dysfunction by Promoting M2 Macrophage Polarization. Oxid Med Cell Longev 2022:3327583 (2022). PubMed: 36285297