Anti-Nicotinic Acetylcholine Receptor beta 2 antibody [270] (ab24698)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor beta 2 antibody [270]
    See all Nicotinic Acetylcholine Receptor beta 2 primary antibodies
  • Description
    Rat monoclonal [270] to Nicotinic Acetylcholine Receptor beta 2
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken
  • Immunogen

    Purified neuronal Nicotinic Acetylcholine Receptors from chicken brain.

  • EpitopeThis clone binds to the extracellular surface of neuronal Acetylcholine Receptor beta 2-subunits.
  • General notes


    This antibody binds to native subunits as well as denatured subunits although with lower affinity. Clone 270 is useful for immunoisolating and pharmacologically characterizing AChRs, immunopurification of AChRs, and is also useful histologically.

Properties

Applications

Our Abpromise guarantee covers the use of ab24698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/150.
WB 1/1000. Predicted molecular weight: 57 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 19176801

Target

  • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in diseaseDefects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor beta 2 neural antibody
    • ACHB2_HUMAN antibody
    • ACHN antibody
    • AChR antibody
    • Acrb 2 antibody
    • Acrb2 antibody
    • b2 nAchR antibody
    • Cholinergic receptor nicotinic beta 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 neuronal antibody
    • cholinergic receptor, nicotinic, beta 2 (neuronal) antibody
    • Chrnb2 antibody
    • EFNL 3 antibody
    • EFNL3 antibody
    • nAChRB2 antibody
    • Neuronal acetylcholine receptor protein beta 2 chain precursor antibody
    • Neuronal acetylcholine receptor protein subunit beta 2 antibody
    • Neuronal acetylcholine receptor subunit beta-2 antibody
    • Neuronal nicotinic acetylcholine receptor beta 2 antibody
    see all

References for Anti-Nicotinic Acetylcholine Receptor beta 2 antibody [270] (ab24698)

This product has been referenced in:
  • Liu Q  et al. A novel nicotinic acetylcholine receptor subtype in basal forebrain cholinergic neurons with high sensitivity to amyloid peptides. J Neurosci 29:918-29 (2009). ICC/IF ; Xenopus laevis . Read more (PubMed: 19176801) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. We do not have a recommended positive control for this antiserum. However, given that this antiserum recognizes Nicotinic Acetylcholine Receptor I would recommend rat brain lysate for use as a positive control for wester...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"