Anti-Nicotinic Acetylcholine Receptor beta 2 antibody (ab55980)

Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor beta 2 antibody
    See all Nicotinic Acetylcholine Receptor beta 2 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta 2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Cat, Pig
  • Immunogen

    A region within synthetic peptide: TEIDLVLKSE VASLDDFTPS GEWDIVALPG RRNENPDDST YVDITYDFII, corresponding to internal sequence amino acids 181-230 of Human Nicotinic Acetylcholine Receptor beta 2

  • Positive control
    • Jurkat cell lysate and human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab55980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease
    Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor beta 2 neural antibody
    • ACHB2_HUMAN antibody
    • ACHN antibody
    • AChR antibody
    • Acrb 2 antibody
    • Acrb2 antibody
    • b2 nAchR antibody
    • Cholinergic receptor nicotinic beta 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 neuronal antibody
    • cholinergic receptor, nicotinic, beta 2 (neuronal) antibody
    • Chrnb2 antibody
    • EFNL 3 antibody
    • EFNL3 antibody
    • nAChRB2 antibody
    • Neuronal acetylcholine receptor protein beta 2 chain precursor antibody
    • Neuronal acetylcholine receptor protein subunit beta 2 antibody
    • Neuronal acetylcholine receptor subunit beta-2 antibody
    • Neuronal nicotinic acetylcholine receptor beta 2 antibody
    see all

Images

  • Anti-Nicotinic Acetylcholine Receptor beta 2 antibody (ab55980) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Nicotinic Acetylcholine Receptor beta 2 with ab55980 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubual. Magnification: 400X.

References

This product has been referenced in:
  • Chen N  et al. An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity. Nat Neurosci 18:892-902 (2015). IHC . Read more (PubMed: 25915477) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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