Anti-Nicotinic Acetylcholine Receptor beta 2 antibody - Carboxyterminal end (ab41174)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab41174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC 1/200.

Target

  • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in diseaseDefects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor beta 2 neural antibody
    • ACHB2_HUMAN antibody
    • ACHN antibody
    • AChR antibody
    • Acrb 2 antibody
    • Acrb2 antibody
    • b2 nAchR antibody
    • Cholinergic receptor nicotinic beta 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 neuronal antibody
    • cholinergic receptor, nicotinic, beta 2 (neuronal) antibody
    • Chrnb2 antibody
    • EFNL 3 antibody
    • EFNL3 antibody
    • nAChRB2 antibody
    • Neuronal acetylcholine receptor protein beta 2 chain precursor antibody
    • Neuronal acetylcholine receptor protein subunit beta 2 antibody
    • Neuronal acetylcholine receptor subunit beta-2 antibody
    • Neuronal nicotinic acetylcholine receptor beta 2 antibody
    see all

Anti-Nicotinic Acetylcholine Receptor beta 2 antibody - Carboxyterminal end images

  • ICC/IF image of ab41174 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41174, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Nicotinic Acetylcholine Receptor beta 2 antibody - Carboxyterminal end (ab41174) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Nicotinic Acetylcholine Receptor beta 2 antibody - Carboxyterminal end (ab41174)

ab41174 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - 3% Triton X100
Specification Brain
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 0.5µg/mL · Temperature: 23°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 15 2015

Application Western blot
Sample Mouse Tissue lysate - whole (brain sample)
Loading amount 10 µg
Specification brain sample
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. M Vandesquille

Verified customer

Submitted Dec 02 2010

Thank you for your enquiry. I have added two of the three following articles using this antibody to our datasheet (I have included a datasheet link below for your convenience). The 1997 article is not available via the internet, but is the original ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"