Anti-Nicotinic Acetylcholine Receptor beta 4 antibody (ab41176)

Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor beta 4 antibody
    See all Nicotinic Acetylcholine Receptor beta 4 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta 4
  • Specificity
    80 % cross reactivity with the full lenght Nicotinic ß4 receptor
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    EGPYAAQRD

    , corresponding to C terminal amino acids 490-498 of Human Nicotinic Acetylcholine Receptor beta 4 attached onto a carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab41176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/200.

Target

  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-4/CHRNB4 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • acetylcholine receptor, nicotinic, beta 4 (neuronal) antibody
    • ACHB4_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor nicotinic beta 4 antibody
    • Cholinergic receptor nicotinic beta polypeptide 4 antibody
    • cholinergic receptor, nicotinic, beta polypeptide 4 antibody
    • Chrnb4 antibody
    • Neuronal acetylcholine receptor subunit beta-4 antibody
    • Neuronal nicotinic receptor beta 4 subunit antibody
    see all

Images

  • ICC/IF image of ab41176 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41176, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab41176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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