Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (ab126234)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta/CHRNB1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody
See all Nicotinic Acetylcholine Receptor beta/CHRNB1 primary antibodies -
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta/CHRNB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor beta/CHRNB1 aa 274-466.
Database link: P11230 -
Positive control
- NT2D1, IMR32, MCF7 and U-87 MG whole cell lysates; H661 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
|
|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Involvement in disease
Defects in CHRNB1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
Defects in CHRNB1 are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (ACHRDCMS) [MIM:608931]. ACHRDCMS is a post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 282179 Cow
- Entrez Gene: 1140 Human
- Entrez Gene: 11443 Mouse
- Entrez Gene: 24261 Rat
- GenBank: NP_000738.2 Human
- Omim: 100710 Human
- SwissProt: P04758 Cow
- SwissProt: P11230 Human
see all -
Alternative names
- Acetylcholine receptor protein beta chain precursor antibody
- Acetylcholine receptor subunit beta antibody
- ACHB_HUMAN antibody
see all
Images
-
Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (ab126234) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
ab126234, at a 1/250 dilution, staining Nicotinic Acetylcholine Receptor beta/CHRNB1 in paraffin-embedded H661 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab126234 has not yet been referenced specifically in any publications.