Anti-Nicotinic Acetylcholine Receptor beta antibody (ab81982)

Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor beta antibody
    See all Nicotinic Acetylcholine Receptor beta primary antibodies
  • Description
    Goat polyclonal to Nicotinic Acetylcholine Receptor beta
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-QEQEDHDALKED

    , corresponding to internal sequence amino acids 448-459 of Human Nicotinic Acetylcholine Receptor beta (NP_000738.2).

  • Positive control
    • Human brain (cerebellum, hippocampus, frontal cortex) lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab81982 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/1000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • Involvement in disease
      Defects in CHRNB1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
      Defects in CHRNB1 are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (ACHRDCMS) [MIM:608931]. ACHRDCMS is a post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance.
    • Sequence similarities
      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily.
    • Cellular localization
      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acetylcholine receptor protein beta chain precursor antibody
      • Acetylcholine receptor subunit beta antibody
      • ACHB_HUMAN antibody
      • AChR antibody
      • ACHRB antibody
      • Cholinergic receptor nicotinic beta polypeptide 1 (muscle) antibody
      • Chrnb 1 antibody
      • CHRNB antibody
      • chrnb1 antibody
      • CMS1D antibody
      • CMS2A antibody
      • Nicotinic acetylcholine receptor beta subunit precursor antibody
      • SCCMS antibody
      see all

    Images

    • Anti-Nicotinic Acetylcholine Receptor beta antibody (ab81982) at 1 µg/ml + human cerebellum lysate in RIPA buffer at 35 µg

      Predicted band size : 57 kDa
      Observed band size : 57 kDa

    References

    ab81982 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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