Anti-Nicotinic Acetylcholine Receptor epsilon antibody (ab166931)

Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor epsilon antibody
    See all Nicotinic Acetylcholine Receptor epsilon primary antibodies
  • Description
    Goat polyclonal to Nicotinic Acetylcholine Receptor epsilon
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Cow, Human, Pig, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, with a Cysteine residue linker, corresponding to internal sequence amino acids 436-446 of Human Nicotinic Acetylcholine Receptor epsilon (NP_000071.1).

  • Positive control
    • Mouse Fetal Skeletal Muscle lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab166931 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease
    The muscle AChR is the major target antigen in the autoimmune disease myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs.
    Myasthenic syndrome, congenital, 4A, slow-channel
    Myasthenic syndrome, congenital, 4B, fast-channel
    Myasthenic syndrome, congenital, 4C, associated with acetylcholine receptor deficiency
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-subfamily.
  • Cellular localization
    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor subunit epsilon antibody
    • ACHE_HUMAN antibody
    • AChR antibody
    • AchR epsilon subunit antibody
    • ACHRE antibody
    • Cholinergic receptor, nicotinic, epsilon polypeptide antibody
    • Chrne antibody
    • CMS1D antibody
    • CMS1E antibody
    • CMS2A antibody
    • FCCMS antibody
    • SCCMS antibody
    see all

Images

  • Anti-Nicotinic Acetylcholine Receptor epsilon antibody (ab166931) at 1 µg/ml + Mouse Fetal Skeletal Muscle lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    Primary incubation was 1 hour.

References

This product has been referenced in:
  • Seaberg B  et al. Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions. Mol Cell Biol 35:1238-53 (2015). Read more (PubMed: 25605336) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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