Anti-Nicotinic Acetylcholine Receptor epsilon antibody (ab54624)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor epsilon antibody
    See all Nicotinic Acetylcholine Receptor epsilon primary antibodies
  • Description
    Mouse monoclonal to Nicotinic Acetylcholine Receptor epsilon
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 21-131 of Human Nicotinic Acetylcholine Receptor epsilon

Properties

Applications

Our Abpromise guarantee covers the use of ab54624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • Involvement in diseaseThe muscle AChR is the major target antigen in the autoimmune disease myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs.
      Myasthenic syndrome, congenital, 4A, slow-channel
      Myasthenic syndrome, congenital, 4B, fast-channel
      Myasthenic syndrome, congenital, 4C, associated with acetylcholine receptor deficiency
    • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-subfamily.
    • Cellular localizationCell junction, synapse, postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acetylcholine receptor subunit epsilon antibody
      • ACHE_HUMAN antibody
      • AChR antibody
      • AchR epsilon subunit antibody
      • ACHRE antibody
      • Cholinergic receptor, nicotinic, epsilon polypeptide antibody
      • Chrne antibody
      • CMS1D antibody
      • CMS1E antibody
      • CMS2A antibody
      • FCCMS antibody
      • SCCMS antibody
      see all

    Anti-Nicotinic Acetylcholine Receptor epsilon antibody images

    • Western blot against tagged recombinant protein immunogen using ab54624 Nicotinic Acetylcholine Receptor epsilon antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-Nicotinic Acetylcholine Receptor epsilon antibody (ab54624)

    ab54624 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - nuclear (hek cell transfected with AChR and human muscle)
    Gel Running Conditions Reduced Denaturing (1%)
    Loading amount 20 µg
    Specification hek cell transfected with AChR and human muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Pedro Cruz

    Verified customer

    Submitted Jun 08 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"