Anti-Nicotinic Acetylcholine Receptor gamma antibody [C9] (ab11151)

Overview

  • Product nameAnti-Nicotinic Acetylcholine Receptor gamma antibody [C9]
    See all Nicotinic Acetylcholine Receptor gamma primary antibodies
  • Description
    Mouse monoclonal [C9] to Nicotinic Acetylcholine Receptor gamma
  • Specificityab11151 binds to Human AChR - gamma subunit found only in foetal AChR. Exhibits homogenous, high avidity binding to the receptor. Binds human recombinant gamma subunit on Western blots.
  • Tested applicationsSuitable for: IHC-Fr, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Chicken
  • Immunogen

    Full length protein (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAscites
  • ClonalityMonoclonal
  • Clone numberC9
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/3.
IP 1/100.
WB 1/100. Predicted molecular weight: 62.4 kDa.
Flow Cyt 1/1. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in diseaseDefects in CHRNG are a cause of multiple pterygium syndrome lethal type (MUPSL) [MIM:253290]. Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent.
    Defects in CHRNG are a cause of multiple pterygium syndrome Escobar variant (MUPSE) [MIM:265000]; also known as nonlethal type multiple pterygium syndrome. Escobar syndrome is a non-lethal form of arthrogryposis multiplex congenita. It is an autosomal recessive condition characterized by excessive webbing (pterygia), congenital contractures (arthrogryposis), and scoliosis. Variable other features include intrauterine death, congenital respiratory distress, short stature, faciocranial dysmorphism, ptosis, low-set ears, arachnodactyly and cryptorchism in males. Congenital contractures are common and may be caused by reduced fetal movements at sensitive times of development. Possible causes of decreased fetal mobility include space constraints such as oligohydramnion, drugs, metabolic conditions or neuromuscular disorders including myasthenia gravis. is a.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Gamma/CHRNG sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor muscle gamma subunit antibody
    • Acetylcholine receptor protein gamma chain precursor antibody
    • Acetylcholine receptor subunit gamma antibody
    • ACHG antibody
    • ACHG_HUMAN antibody
    • Achr 3 antibody
    • AChR antibody
    • Achr3 antibody
    • ACHRG antibody
    • ACRG antibody
    • Cholinergic receptor nicotinic gamma antibody
    • Cholinergic receptor nicotinic gamma polypeptide antibody
    • CHRNG antibody
    • MGC133376 antibody
    see all

References for Anti-Nicotinic Acetylcholine Receptor gamma antibody [C9] (ab11151)

ab11151 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Non-reduced Denaturing (10% gel BioRad, Running Buffer BioRad)
Sample Mouse Tissue lysate - whole (tibialis muscle)
Specification tibialis muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Ms. Lisa Marienhoff

Verified customer

Submitted Nov 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"