Overview

  • Product name
  • Description
    Rabbit polyclonal to Nidogen 2
  • Tested applications
    Suitable for: WB, ELISA, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab14513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 153 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23211522ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200. PubMed: 23409002

Target

  • Function
    Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
  • Tissue specificity
    Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
  • Sequence similarities
    Contains 5 EGF-like domains.
    Contains 5 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 2 thyroglobulin type-1 domains.
  • Post-translational
    modifications
    Highly N- and O-glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • NID 2 antibody
    • NID-2 antibody
    • Nid2 antibody
    • NID2_HUMAN antibody
    • Nidogen-2 antibody
    • Nidogen2 antibody
    • Osteonidogen antibody
    see all

References

This product has been referenced in:
  • Murphy PA  et al. Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors. PLoS One 10:e0120872 (2015). Read more (PubMed: 25807551) »
  • Torricelli AA  et al. Epithelial basement membrane proteins perlecan and nidogen-2 are up-regulated in stromal cells after epithelial injury in human corneas. Exp Eye Res 134:33-8 (2015). Read more (PubMed: 25797478) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (retinal tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Heat mediated antigen retrieval solution pH: 6
Permeabilization
Yes - 0.1% Tween 20
Specification
retinal tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Metanol 95% C/ Acético Glacial 5%
Username

Dr. Patricia Bogdanov

Verified customer

Submitted Jul 18 2016

Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiser...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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