Recombinant
RabMAb

Anti-Niemann Pick C1 Like 1 antibody [EPR5717] (ab124801)

Overview

  • Product name
    Anti-Niemann Pick C1 Like 1 antibody [EPR5717]
    See all Niemann Pick C1 Like 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Niemann Pick C1 Like 1 aa 1300 to the C-terminus.

  • Positive control
    • Human fetal liver and HeLa lysates; Human liver tissue.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism.
    • Tissue specificity
      Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle.
    • Sequence similarities
      Belongs to the patched family.
      Contains 1 SSD (sterol-sensing) domain.
    • Post-translational
      modifications
      Highly glycosylated.
    • Cellular localization
      Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane. Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5.
    • Information by UniProt
    • Database links
    • Alternative names
      • Niemann Pick C1 like protein 1 antibody
      • Niemann Pick disease type C1 gene like 1 antibody
      • Niemann-Pick C1-like protein 1 antibody
      • NPC1 LIKE 1 antibody
      • NPC1 Niemann Pick disease type C1 gene like 1 antibody
      • NPC1L1 antibody
      • NPC1L1 splice variant antibody
      • NPCL1_HUMAN antibody
      • Truncated Niemann Pick C1 like protein 1 antibody
      see all

    Images

    • Anti-Niemann Pick C1 Like 1 antibody [EPR5717] (ab124801) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 149 kDa
      Observed band size : 149 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Niemann Pick C1 Like 1 antibody [EPR5717] (ab124801) at 1/10000 dilution (purified) + Human fetal liver lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 149 kDa
      Observed band size : 149 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Niemann Pick C1 Like 1 antibody [EPR5717] (ab124801) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver lysates
      Lane 2 : HeLa lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 149 kDa

    References

    This product has been referenced in:
    • Hu YW  et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014). Read more (PubMed: 24493833) »
    • Renner O  et al. Role of the ABCG8 19H risk allele in cholesterol absorption and gallstone disease. BMC Gastroenterol 13:30 (2013). WB . Read more (PubMed: 23406058) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells over expressing human NPC1L1)
    Loading amount
    100 µg
    Specification
    HEK293T cells over expressing human NPC1L1
    Gel Running Conditions
    Reduced Denaturing (7.5% Tris-Glycine gel)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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