Anti-Niemann Pick C2 antibody (ab92736)
Key features and details
- Rabbit polyclonal to Niemann Pick C2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Niemann Pick C2 antibody
See all Niemann Pick C2 primary antibodies -
Description
Rabbit polyclonal to Niemann Pick C2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Monkey -
Immunogen
Synthetic peptide corresponding to Human Niemann Pick C2 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab93309) -
Positive control
- This antibody gave a positive signal in human testis tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
May be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis. -
Tissue specificity
Epididymis. -
Involvement in disease
Defects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2) [MIM:607625]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. -
Sequence similarities
Belongs to the NPC2 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 450192 Chimpanzee
- Entrez Gene: 10577 Human
- Omim: 601015 Human
- SwissProt: P61917 Chimpanzee
- SwissProt: P61916 Human
- SwissProt: P61918 Monkey
- Unigene: 433222 Human
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Alternative names
- EDDM1 antibody
- Epididymal protein 1 antibody
- Epididymal secretory protein antibody
see all
Images
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Anti-Niemann Pick C2 antibody (ab92736) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Human Epididymal secretory protein E1 precursor (Niemann pick C2)contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
IHC image of Niemann Pick C2 staining in human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 92736, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab92736 has not yet been referenced specifically in any publications.