The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceNIF1 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. This protein may function by altering local chromatin structure.
Detection of human NIF1 by Immunohistochemistry. Sample: formalin fixed, paraffin embedded section of human non small cell lung cancer.Antibody: ab84551 used at a dilution of 1/250. Detection: DAB staining.
Detection of mouse NIF1 by Immunohistochemistry. Sample: formalin fixed, paraffin embedded section section of mouse squamous cell carcinoma.Antibody: ab84551 used at a dilution of 1/250. Detection: DAB staining.
References for Anti-NIF1 antibody (ab84551)
has not yet been referenced specifically in any publications.
Publishing research using ab84551? Please let us know so that we can cite the reference in this datasheet.
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