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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab61203 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
1/100 - 1/500.
1/50 - 1/100.
Sequence similarities Contains 1 RRM (RNA recognition motif) domain.
Post-translational modifications Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Cellular localization Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
Information by UniProt
MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
Anti-NIFK antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NIFK antibody (ab61203)
ab61203 at 1/50 dilution staining NIFK in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - NIFK antibody (ab61203)
ab61203 at 1/100 dilution staining NIFK in HuvEc cells by Immunofluorescence, in the absence (left) or presence (right) of the immunising peptide.
Western blot - NIFK antibody (ab61203)
All lanes : Anti-NIFK antibody (ab61203) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate ( ab76999) with SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) ( ab65484) at 1/3000 dilution Predicted band size : 34 kDa Observed band size : 41 kDa ( why is the actual band size different from the predicted?)
References for Anti-NIFK antibody (ab61203)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"