Overview

  • Product nameAnti-NIFK (phospho T234) antibody
    See all NIFK primary antibodies
  • Description
    Rabbit polyclonal to NIFK (phospho T234)
  • Specificityab61110 detects endogenous levels of NIFK only when phosphorylated at threonine 234.
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from human NIFK around the phosphorylation site of threonine 234 (G-P-TP-P-V).

  • Positive control
    • Human brain tissue and HuvEC cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

Target

  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
  • Cellular localizationNucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
  • Information by UniProt
  • Database links
  • Alternative names
    • hNIFK antibody
    • MK67I_HUMAN antibody
    • MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
    • MKI67 FHA domain-interacting nucleolar phosphoprotein antibody
    • mki67ip antibody
    • NOPP34 antibody
    • Nucleolar phosphoprotein Nopp34 antibody
    • Nucleolar protein interacting with the FHA domain of pKI 67 antibody
    • Nucleolar protein interacting with the FHA domain of pKI-67 antibody
    • pKi67 antibody
    see all

Anti-NIFK (phospho T234) antibody images

  • ab61110 at a 1/50-1/100 dilution, staining human NIFK in brain tissue with (+) or without (-) immunising phospopeptide, using Immunohistochemistry, Paraffin embedded tissue
  • ab61110, at a 1/100-1/500 dilution, staining human NIFK in HuvEC cells with (+) or without (-) immunising phospopeptide, using Immunofluorescence

References for Anti-NIFK (phospho T234) antibody (ab61110)

This product has been referenced in:
  • Booth DG  et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:e01641 (2014). ICC/IF ; Human . Read more (PubMed: 24867636) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab61110.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"