Overview

  • Product nameNigericin Na+ salt
  • Description
    K+ ionophore
  • Biological descriptionK+ ionophore; exchanges K+ for H+ across most biological membranes. Stimulates mitochondrial ATPase activity, and exchanges H+ for K+>Rb+>Cs+>>Na+. Reported to inhibit Poliovirus and influenza virus replication.
  • Purity> 98%

Properties

  • Chemical nameNigericin sodium salt
  • Molecular weight746.95
  • Chemical structureChemical Structure
  • Molecular formulaC40H67NaO11
  • CAS Number28643-80-3
  • PubChem identifier16760591
  • Storage instructionsStore at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overviewSoluble in ethanol to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESOC[C@]1(O)O[C@@H]([C@@H](C)C[C@H]1C)[C@H]2C[C@H](C)[C@@H](O2)[C@]3(C)CC[C@@H](O3)[C@]6(C)C[C@@H](C)[C@@]5(O[C@H](C[C@H]4CC[C@H](C)[C@@H](O4)[C@@H](C)C(=O)O[Na])C[C@@H](OC)[C@H]5C)O6
  • Source

    Streptomyces hygroscopicus

  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab120494 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Nigericin Na+ salt images

    • ab56889 staining mitofusin 2 in MEF1 cells treated with nigericin Na+ salt (ab120494), by ICC/IF. Decrease in mitofusin 2 expression correlates with increased concentration of nigericin Na+ salt, as described in literature.
      The cells were incubated at 37°C for 3h in media containing different concentrations of ab120494 (nigericin Na+ salt) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Nigericin Na+ salt (ab120494)

    This product has been referenced in:
    • Myskiw C  et al. Nigericin is a potent inhibitor of the early stage of vaccinia virus replication. Antiviral Res 88:304-10 (2010). Read more (PubMed: 20951746) »
    • Margolis LB  et al. K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells. Proc Natl Acad Sci U S A 86:6626-9 (1989). Read more (PubMed: 2771947) »
    • Daniele RP  et al. A potassium ionophore (Nigericin) inhibits stimulation of human lymphocytes by mitogens. J Exp Med 147:571-81 (1978). Read more (PubMed: 146727) »

    See 0 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"