NIH 3T3 cytoplasmic extract lysate (ab14873)

Overview

  • Product nameNIH 3T3 cytoplasmic extract lysate
    See all NIH 3T3 lysates
  • General notesCell line: NIH/3T3 (Mouse embryonic fibroblast).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • BackgroundNIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies

References for NIH 3T3 cytoplasmic extract lysate (ab14873)

ab14873 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call and I apologize for the shortage of ab14873 that you received. The free of charge replacement vial is being sent to your attention on order reference # 133497. The item is currently out of stock but you will receive email ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"