Anti-Ninein antibody - Aminoterminal end (ab52473)

Overview

  • Product nameAnti-Ninein antibody - Aminoterminal end
    See all Ninein primary antibodies
  • Description
    Rabbit polyclonal to Ninein - Aminoterminal end
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Ninein (N terminal).

  • Positive control
    • MCF7, MOLT-4, HOS, NIH3T3, HeLa and MCF-7 cell line extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab52473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 200 kDa.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Centrosome Marker antibody
    • Glycogen synthase kinase 3 beta interacting protein antibody
    • GSK3B interacting protein antibody
    • h Glycogen synthase kinase 3 beta-interacting protein antibody
    • hNinein antibody
    • KIAA1565 antibody
    • NIN antibody
    • NIN protein antibody
    • NIN_HUMAN antibody
    • ninein (GSK3B interacting protein) antibody
    • Ninein antibody
    • Ninein centrosomal protein antibody
    • SCKL7 antibody
    see all

Anti-Ninein antibody - Aminoterminal end images

  • All lanes : Anti-Ninein antibody - Aminoterminal end (ab52473) (10 µl per 5 ml antibody dilution buffer for each mini-gel.)

    Lane 1 : HOS cell lysate
    Lane 2 : NIH3T3 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF-7 cell lysate
  • All lanes : Anti-Ninein antibody - Aminoterminal end (ab52473) at 1/500 dilution

    Lane 1 : MCF7 cell line extract
    Lane 2 : MOLT4 cell line extract

    Secondary
    HRP conjugated donkey anti-rabbit
    Developed using the ECL technique

    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,130 kDa. We are unsure as to the identity of these extra bands.
    Additional bands are observed only in hematopoietic cells (such as MOLT-4).

References for Anti-Ninein antibody - Aminoterminal end (ab52473)

ab52473 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Dog Cell (MDCK-2 kidney epithelial)
Specification MDCK-2 kidney epithelial
Permeabilization No
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted May 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mIMCD-3 kidney epithelial cell line)
Specification mIMCD-3 kidney epithelial cell line
Fixative Methanol
Permeabilization Yes - NP40
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Benjamin Tyrrell

Verified customer

Submitted May 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (ARPE-19 cells)
Specification ARPE-19 cells
Fixative Methanol
Permeabilization Yes - NP40
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Benjamin Tyrrell

Verified customer

Submitted May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"