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Fusion protein containing amino acids 32-506 of murine Ninein
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
Our Abpromise guarantee covers the use of ab4447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 28469279|
|WB||1/500. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).|
IF using ab4447 to detect Ninein in MCF7 cells (human).
The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein.
ab4447 was used at 1/1000.
Ninein shows nuclear and centrosome staining, which is expected from the literature.
ICC/IF image of ab4447 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4447, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab4447 shows the characteristic nuclear and centrosome staining for Ninein.
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