Overview

  • Product name
  • Description
    Rabbit polyclonal to NIP7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 130-180 of Human NIP7 (NP_057185.1).

  • Positive control
    • HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
  • Tissue specificity
    Expressed in hematopoietic stem/progenitor cells.
  • Sequence similarities
    Belongs to the NIP7 family.
    Contains 1 PUA domain.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosome subunit biogenesis protein NIP7 homolog antibody
    • CGI 37 antibody
    • CGI37 antibody
    • FLJ10296 antibody
    • HSPC031 antibody
    • HSPC180 antibody
    • KD93 antibody
    • NIP 7 antibody
    • NIP7 antibody
    • NIP7 nucleolar pre rRNA processing protein antibody
    • NIP7_HUMAN antibody
    • Nuclear import 7 antibody
    • Nuclear import 7 homolog (S. cerevisiae) antibody
    • Nuclear import 7 homolog antibody
    • OK/SW cl.76 antibody
    • OK/SW cl.78 antibody
    • OTTHUMP00000174889 antibody
    see all

Images

  • All lanes : Anti-NIP7 antibody (ab168814) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 20 kDa


    Exposure time: 3 minutes
  • Detection of NIP7 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168814 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 shows control IgG IP.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab168814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168814.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up