Overview

  • Product name
  • Description
    Rabbit polyclonal to NIPA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 27-261 of NIPA (Uniprot ID Q86WB0)

  • Positive control
    • 293T, HeLa, HepG2 and mouse brain whole cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
  • Tissue specificity
    Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 C3HC-type zinc finger.
  • Developmental stage
    Weakly expressed in G0/G1 phases, abundant during S and G2/M phases, and strongly decreases thereafter.
  • Domain
    The F-box-like region is required for the interaction with SKP1.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hNIPA antibody
    • NIPA_HUMAN antibody
    • Nuclear interacting partner of ALK antibody
    • Nuclear interacting partner of anaplastic lymphoma kinase antibody
    • Nuclear-interacting partner of ALK antibody
    • Nuclear-interacting partner of anaplastic lymphoma kinase antibody
    • zc3hc1 antibody
    • Zinc finger C3HC type containing 1 antibody
    • Zinc finger C3HC-type protein 1 antibody
    see all

Images

  • Anti-NIPA antibody (ab154553) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Anti-NIPA antibody (ab154553) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 55 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling NIPA with ab154553 antibody at 1/200 dilution. Bottom figure: staining merged with DNA probe.

References

ab154553 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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