Anti-NIPA (phospho S354) antibody (ab63557)
Key features and details
- Rabbit polyclonal to NIPA (phospho S354)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-NIPA (phospho S354) antibody
See all NIPA primary antibodies -
Description
Rabbit polyclonal to NIPA (phospho S354) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human NIPA (phospho S354).
Database link: Q86WB0 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. -
Tissue specificity
Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 C3HC-type zinc finger. -
Developmental stage
Weakly expressed in G0/G1 phases, abundant during S and G2/M phases, and strongly decreases thereafter. -
Domain
The F-box-like region is required for the interaction with SKP1. -
Post-translational
modificationsPhosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 232679 Mouse
- Entrez Gene: 296957 Rat
- SwissProt: Q80YV2 Mouse
- Unigene: 29780 Mouse
- Unigene: 474686 Mouse
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Alternative names
- hNIPA antibody
- NIPA_HUMAN antibody
- Nuclear interacting partner of ALK antibody
see all
Images
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All lanes : Anti-NIPA (phospho S354) antibody (ab63557) at 1/500 dilution
Lane 1 : extracts from COS7 cells, treated
with HU (2nM, 24hours)
Lane 2 : extracts from COS7 cells, treated
with HU (2nM, 24hours) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab63557 has been referenced in 2 publications.
- Zhao M et al. Methylene blue exerts a neuroprotective effect against traumatic brain injury by promoting autophagy and inhibiting microglial activation. Mol Med Rep 13:13-20 (2016). WB ; Mouse . PubMed: 26572258
- Illert AL et al. Extracellular signal-regulated kinase 2 (ERK2) mediates phosphorylation and inactivation of nuclear interaction partner of anaplastic lymphoma kinase (NIPA) at G2/M. J Biol Chem 287:37997-8005 (2012). WB . PubMed: 22955283