Anti-NIPA1 antibody (ab121744)
Key features and details
- Rabbit polyclonal to NIPA1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NIPA1 antibody -
Description
Rabbit polyclonal to NIPA1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant Protein Epitope Signature Tag, corresponding to amino acids 183-226 (HGPTNIMVYI SICSLLGSFT VPSTKGIGLA AQDILHNNPS SQRA) of Human NIPA1.
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Positive control
- Human urinary bladder tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+). -
Tissue specificity
Widely expressed with highest levels in neuronal tissues. -
Involvement in disease
Defects in NIPA1 are the cause of spastic paraplegia autosomal dominant type 6 (SPG6) [MIM:600363]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. -
Sequence similarities
Belongs to the NIPA family. -
Cellular localization
Cell membrane. Early endosome. Recruited to the cell membrane in response to low extracellular magnesium. - Information by UniProt
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Database links
- Entrez Gene: 123606 Human
- Omim: 608145 Human
- SwissProt: Q7RTP0 Human
- Unigene: 511797 Human
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Alternative names
- FSP 3 antibody
- FSP3 antibody
- Magnesium transporter NIPA1 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121744 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121744, at a 1/10 dilution, staining NIPA1 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121744 has not yet been referenced specifically in any publications.