Overview

  • Product nameAnti-NIPA1 antibody
    See all NIPA1 primary antibodies
  • Description
    Rabbit polyclonal to NIPA1
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag, corresponding to amino acids 183-226 (HGPTNIMVYI SICSLLGSFT VPSTKGIGLA AQDILHNNPS SQRA) of Human NIPA1.

  • Positive control
    • Human urinary bladder tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActs as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).
  • Tissue specificityWidely expressed with highest levels in neuronal tissues.
  • Involvement in diseaseDefects in NIPA1 are the cause of spastic paraplegia autosomal dominant type 6 (SPG6) [MIM:600363]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
  • Sequence similaritiesBelongs to the NIPA family.
  • Cellular localizationCell membrane. Early endosome. Recruited to the cell membrane in response to low extracellular magnesium.
  • Information by UniProt
  • Database links
  • Alternative names
    • FSP 3 antibody
    • FSP3 antibody
    • Magnesium transporter NIPA1 antibody
    • MGC102724 antibody
    • MGC35570 antibody
    • NIPA 1 antibody
    • NIPA1 antibody
    • NIPA1_HUMAN antibody
    • Non imprinted in Prader Willi/Angelman syndrome region protein 1 antibody
    • Non-imprinted in Prader-Willi/Angelman syndrome region protein 1 antibody
    • Spastic paraplegia 6 (autosomal dominant) antibody
    • Spastic paraplegia 6 protein antibody
    • SPG 6 antibody
    • SPG6 antibody
    see all

Anti-NIPA1 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121744 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121744, at a 1/10 dilution, staining NIPA1 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.

References for Anti-NIPA1 antibody (ab121744)

ab121744 has not yet been referenced specifically in any publications.

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