Overview

  • Product nameAnti-NIPP1 antibody
    See all NIPP1 primary antibodies
  • Description
    Goat polyclonal to NIPP1
  • Tested applicationsSuitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: EAWPGKKPTPSLLI, corresponding to C terminal amino acids 114-127 of Human NIPP1.

  • General notes


    This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2.5 - 3.8 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human NIPP1 peptide (ab23096).

Target

  • FunctionInhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
    Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • Tissue specificityUbiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
  • Sequence similaritiesContains 1 FHA domain.
  • DomainHas a basic N- and C-terminal and an acidic central domain.
  • Post-translational
    modifications
    May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
  • Cellular localizationNucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of RNA decay antibody
    • ARD 1 antibody
    • ARD-1 antibody
    • ARD1 antibody
    • Homolog of E.coli RNase E antibody
    • NIPP 1 antibody
    • NIPP-1 antibody
    • NIPP1 antibody
    • Nuclear inhibitor of protein phosphatase 1 antibody
    • nuclear inhibitor of protein phosphatase-1 alpha antibody
    • Nuclear subunit of PP1 antibody
    • PP1R8_HUMAN antibody
    • PPP1R8 antibody
    • PRO2047 antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 8 antibody
    • Protein phosphatase 1 regulatory subunit 8 antibody
    • RNase E antibody
    • RNase E, E. coli, homolog of antibody
    see all

Anti-NIPP1 antibody images



  • Predicted band size : 40 kDa


    ab5300 at a concentration of 2µg/ml staining approximately 40 kDa human NIPP 1 in H460 cell lysate (30µg total protein per lane) by Western blot (ECL).
  • ab5300(3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-NIPP1 antibody (ab5300)

This product has been referenced in:
  • Nuytten M  et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . Read more (PubMed: 17724462) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"