• Product nameAnti-NIPP1 antibody
    See all NIPP1 primary antibodies
  • Description
    Rabbit polyclonal to NIPP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 216-265 (DPSVGRFRNM VQTAVVPVKK KRVEGPGSLG LEESGSRRMQ NFAFSGGLYG) of human PPP1R8 (NP_054829).

  • Positive control
    • 293T cell lysate



Our Abpromise guarantee covers the use of ab86352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionInhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
    Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • Tissue specificityUbiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
  • Sequence similaritiesContains 1 FHA domain.
  • DomainHas a basic N- and C-terminal and an acidic central domain.
  • Post-translational
    May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
  • Cellular localizationNucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of RNA decay antibody
    • ARD 1 antibody
    • ARD-1 antibody
    • ARD1 antibody
    • Homolog of E.coli RNase E antibody
    • NIPP 1 antibody
    • NIPP-1 antibody
    • NIPP1 antibody
    • Nuclear inhibitor of protein phosphatase 1 antibody
    • nuclear inhibitor of protein phosphatase-1 alpha antibody
    • Nuclear subunit of PP1 antibody
    • PP1R8_HUMAN antibody
    • PPP1R8 antibody
    • PRO2047 antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 8 antibody
    • Protein phosphatase 1 regulatory subunit 8 antibody
    • RNase E antibody
    • RNase E, E. coli, homolog of antibody
    see all

Anti-NIPP1 antibody images

  • Anti-NIPP1 antibody (ab86352) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 55 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NIPP1 antibody (ab86352)

ab86352 has not yet been referenced specifically in any publications.

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