The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 5 µg/ml.
Peptide ELISA: antibody detection limit dilution 1:16,000.
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Please note that currently we cannot find an explanation in the literature for the band we observed at size of 107kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCatalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes (in vitro) (By similarity). Binds calcium ions.
Tissue specificityDetected in brain and spleen, and at low levels in ovary.
Involvement in diseaseDefects in PITPNM3 are the cause of cone-rod dystrophy type 5 (CORD5) [MIM:600977]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
Sequence similaritiesBelongs to the PtdIns transfer protein family. PI transfer class IIA subfamily. Contains 1 DDHD domain.
ab21197 at 5ug/ml staining NIR1 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
References for Anti-NIR1 antibody (ab21197)
has not yet been referenced specifically in any publications.
Publishing research using ab21197? Please let us know so that we can cite the reference in this datasheet.
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