Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab22823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notesICC: 1/200 - please refer to abreview for further details.
    WB: See Abreview published on 11th April, 2007 for further information.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the PtdIns transfer protein family. PI transfer class IIA subfamily.
      Contains 1 DDHD domain.
    • Post-translational
      modifications
      Phosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1.
      Phosphorylated on threonine residues upon treatment with oleic acid.
      Phosphorylated on tyrosine residues by PTK2B.
    • Cellular localizationCytoplasm. Golgi apparatus > Golgi stack membrane. Endoplasmic reticulum membrane. Lipid droplet. Cleavage furrow. Midbody. Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • DRES9 antibody
      • Drosophila retinal degeneration B antibody
      • Drosophila retinal degeneration B homolog antibody
      • FLJ44997 antibody
      • membrane-associated 1 antibody
      • Membrane-associated phosphatidylinositol transfer protein 1 antibody
      • NIR 2 antibody
      • NIR-2 antibody
      • NIR2 antibody
      • OTTHUMP00000236414 antibody
      • OTTHUMP00000236415 antibody
      • Phosphatidylinositol transfer protein antibody
      • phosphatidylinositol transfer protein membrane associated antibody
      • PITM1_HUMAN antibody
      • PITPnm 1 antibody
      • PITPNM antibody
      • Pitpnm1 antibody
      • PYK2 N terminal domain interacting receptor 2 antibody
      • Pyk2 N-terminal domain-interacting receptor 2 antibody
      • RD 9 antibody
      • RD9 antibody
      • RDGB antibody
      • RDGB1 antibody
      • RDGBA1 antibody
      • retinal degeneration B alpha 1 antibody
      see all

    Anti-NIR2 antibody images

    • ab22823 (1/200) staining NIR2 in mouse macrophage cells by ICC/IF. Secondary antibody: donkey polyclonal to goat IgG conjugated to Alexa Fluor® 594; Counterstain: DAPI (blue) - please refer to abrevew for further details.

      See Abreview

    References for Anti-NIR2 antibody (ab22823)

    This product has been referenced in:
    • Keinan O  et al. The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis. J Cell Sci 127:4740-9 (2014). Read more (PubMed: 25179602) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (MCF10A)
    Loading amount 50 µg
    Specification MCF10A
    Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 05 2012

    Thank you very much for your reply.


    I am pleased to hear you would like to make use of our testing discount offer to test ab22823NIR2 antibodyin human samples. I can provide the following discount code.

    DISCOUNT CODE: ##### <...

    Read More

    Thank you for your reply

    I confirm can offer a discount off a future purchase if you buy ab22823 now, test it in human samples and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negati...

    Read More

    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware, ab22823 has never been tested with samples from human. All tested species cross-reactivity information covered by our guara...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse macrophage cell line)
    Loading amount 50 µg
    Specification mouse macrophage cell line
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 11 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Mouse Cell (MOUSE MACROPHAGE CELL LINES)
    Specification MOUSE MACROPHAGE CELL LINES
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1.5%
    Username

    Abcam user community

    Verified customer

    Submitted Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"