The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: 1/200 - please refer to abreview for further details.
WB: See Abreview published on 11th April, 2007 for further information.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRegulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
Sequence similaritiesBelongs to the PtdIns transfer protein family. PI transfer class IIA subfamily. Contains 1 DDHD domain.
Post-translational modificationsPhosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1. Phosphorylated on threonine residues upon treatment with oleic acid. Phosphorylated on tyrosine residues by PTK2B.
Cellular localizationCytoplasm. Golgi apparatus > Golgi stack membrane. Endoplasmic reticulum membrane. Lipid droplet. Cleavage furrow. Midbody. Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis.
Immunocytochemistry/ Immunofluorescence - NIR2 antibody (ab22823)This image is courtesy of an anonymous Abreview
ab22823 (1/200) staining NIR2 in mouse macrophage cells by ICC/IF. Secondary antibody: donkey polyclonal to goat IgG conjugated to Alexa Fluor® 594; Counterstain: DAPI (blue) - please refer to abrevew for further details.