Overview

  • Product nameAnti-NIRF antibody
    See all NIRF primary antibodies
  • Description
    Rabbit polyclonal to NIRF
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide derived from residues 186-235 of human NIRF.

  • Positive control
    • Jurkat cell lysate (WB), human heart (IHC-P).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionE3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of PCNP. May participate in methylation-dependent transcriptional regulation. Important for G1/S transition. Overexpression causes G1 phase cell arrest.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.
  • Post-translational
    modifications
    May be autoubiquitinated; which may lead to proteasomal degradation.
    Phosphorylated. Phosphorylation may be mediated by CDK2.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434B0920 antibody
    • DKFZp686G0837 antibody
    • E3 ubiquitin-protein ligase UHRF2 antibody
    • MGC33463 antibody
    • Np95 like RING finger protein antibody
    • Np95-like ring finger protein antibody
    • Np95/ICBP90 like RING finger protein antibody
    • Np95/ICBP90-like RING finger protein antibody
    • Nuclear protein 97 antibody
    • Nuclear zinc finger protein Np97 antibody
    • RING finger protein 107 antibody
    • RNF 107 antibody
    • RP11-472F14.2 antibody
    • Ubiquitin like containing PHD and RING finger domains protein 2 antibody
    • Ubiquitin-like PHD and RING finger domain-containing protein 2 antibody
    • Ubiquitin-like-containing PHD and RING finger domains protein 2 antibody
    • Uhrf2 antibody
    • UHRF2_HUMAN antibody
    • URF 2 antibody
    see all

Anti-NIRF antibody images

  • Anti-NIRF antibody (ab28673) at 0.5 µg/ml + Jurkat Cell Lysate with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG1: 50,000 - 100,000 dilution.

    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • IHC staining of paraffin embedded human heart with ab28673 at 4.0-8.0 ug/ml. Positive staining (arrows) is myocardial cells. Magnification: x400

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Western blot analysis using ab28673 at 1/2000.

    Lane 1) Whole cell HeLa lysate
    Lane 2) Recombinant purified UHRF2 protein
    Lane 3) Recombinant purified UHRF1 protein
    Lane 4) Whole cell HeLa lysate with UHRF1 knockdown (short hairpin RNA)

    A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-NIRF antibody (ab28673)

This product has been referenced in:
  • He X  et al. Let-7a elevates p21(WAF1) levels by targeting of NIRF and suppresses the growth of A549 lung cancer cells. FEBS Lett 583:3501-7 (2009). Human . Read more (PubMed: 19818775) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (9%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Sep 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"