Overview

  • Product nameAnti-NISCH antibody
  • Description
    Mouse monoclonal to NISCH
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: LTGSTPMQVV TCLTRDSYLT HCFLQHLMVV LSSLERTPSP EPVDKDFYSE FGNKTTGKME NYELIHSSRV KFTYPSEEEI GDLTFTVAQK MAEPEKAPAL , corresponding to amino acids 1246-1346 of Human NISCH

  • Positive control
    • ICC/IF: SKNSH cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Flow Cyt Use 0.01-0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceMouse Nischarin (NISCH) has been shown to interact with the alpha-5 subunit of integrin and inhibit cell migration. Its human homologue (imidazoline receptor antisera-selected (IRAS)), contains an NH2-terminal extension and is a larger protein of 1504 amino acids consisting of an NH2-terminal PX domain, 5 putative leucine-rich repeats, a predicted coiled-coil domain, and a long COOH-terminal region. It has the ability to homo-oligomerize via its coiled-coil region. The PX domain of IRAS is essential for association with phosphatidylinositol 3-phosphate-enriched endosomal membranes. NISCH may serve as a functional imidazoline I1-receptor.
  • Cellular localizationCell membrane. Cytoplasm. Early endosome. Recycling endosome.
  • Database links
  • Alternative names
    • FLJ14425 antibody
    • FLJ40413 antibody
    • FLJ90519 antibody
    • I 1 antibody
    • I 1 receptor candidate protein antibody
    • I1R candidate protein antibody
    • Imidazoline 1 receptor candidate protein antibody
    • Imidazoline receptor 1 antibody
    • Imidazoline receptor antisera selected antibody
    • Imidazoline receptor antisera selected protein antibody
    • Imidazoline receptor candidate antibody
    • IR1 antibody
    • IRAS antibody
    • KIAA0975 antibody
    • Nischarin antibody
    see all

Anti-NISCH antibody images

  • ICC/IF image of ab56849 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56849, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing SHSY-5Y cells stained with ab56849 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56849, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot against tagged recombinant protein immunogen using ab56849 NISCH antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

References for Anti-NISCH antibody (ab56849)

This product has been referenced in:
  • Chen MF  et al. Characterization of imidazoline receptors in blood vessels for the development of antihypertensive agents. Biomed Res Int 2014:182846 (2014). WB ; Rat . Read more (PubMed: 24800210) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"