MitoSciences (MS1091)

Nitrated Human Liver Homogenate Standard (ab131380)

Overview

  • Product nameNitrated Human Liver Homogenate Standard
  • Tested applicationsWB, SDS-PAGEmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferConstituents: 0.806% Sodium chloride, Proprietary component, 1.5% Dodecyl maltoside
  • Concentration information loading...
  • Reagent notes ab131380 was treated with peroxynitrite which modifies tyrosine by acting as an oxidant to produce 3-nitrotyrosine along proteins in the Human Liver Tissue lysate sample.
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab131380 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration.
    SDS-PAGE Use at an assay dependent concentration.

    Nitrated Human Liver Homogenate Standard images

    • All lanes : Anti-Nitrotyrosine antibody ab110282 at 0.4 µg/ml

      Lane 1 : Molecular weight marker
      Lane 2 : Human Liver Homogenate at 10 µg
      Lane 3 : Human Liver Homogenate at 2.5 µg
      Lane 4 : Nitrated Human Liver Homogenate at 10 µg
      Lane 5 : Nitrated Human Liver Homogenate at 5 µg
      Lane 6 : Nitrated Human Liver Homogenate at 2.5 µg
      Lane 7 : Nitrated Human Liver Homogenate at 1.25 µg
      Lane 8 : Nitrated Human Liver Homogenate at 0.625 µg

      Secondary
      Goat polyclonal to Mouse IgG, Pre-Adsorbed (HRP) at 1/6000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes

    • Lane 1: Molecular Weight Marker
      Lane 2: Nitrated Human Liver Homogenate (10 µg)
      Lane 3: Human Liver Homogenate (10 µg)

      Gel: 10-20% acrylamide separation gel

    References for Nitrated Human Liver Homogenate Standard (ab131380)

    ab131380 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

    We have 5 liver lysates that are from different donors:

    Ab44563-

    http://www.abcam.com/Liver-Human-Tissue-Lysate-adult-n...

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    Thank you for calling Abcam today.

    I am sorry about the issues that you have been having with ab13868 in western blot. AS we discussed today, I am sending you a vial of human liver lysate to use as a positive control (although I am sending ab2...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"