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Abcam's Nitric Oxide Assay Kit (Colorimetric) provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples.
Visit our FAQs page for tips and troubleshooting.
If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327).
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production.
|Assay Buffer||WM||1 x 30ml|
|Enhancer (Lyophilized)||Purple||1 vial|
|Enzyme Cofactor (Lyophilized)||Blue||1 vial|
|Griess Reagent R1||NM||1 x 10ml|
|Griess Reagent R2||Amber NM||1 x 10ml|
|Microtiter plate + Plate Cover||2 units|
|Nitrate Reductase (Lyophilized)||Green||1 vial|
|Nitrate Standard (Lyophilized)||Yellow||1 vial|
|Nitrite Standard (Lyophilized)||Orange||1 vial|
Our Abpromise guarantee covers the use of ab65328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Nitrite and nitrate measured in cell lysates showing quantity (micromol) per 106 cells
Nitrite and nitrate measured in biologicals showing concentration (micromolar)
Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
Standard curve (Colorimetric) : mean of duplicates (+/-SD) with background readings subtracted