Overview

  • Product name
    Anti-Nitro tyrosine antibody
    See all Nitro tyrosine primary antibodies
  • Description
    Rabbit polyclonal to Nitro tyrosine
  • Specificity
    This antibody reacts against Nitrotyrosine, but with less than 10% reactivity against chlorotyrosine.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Nitrotyrosine containing synthetic peptide.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, Tris buffered saline, 0.5mg/ml BSA, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    This antibody was prepared by affinity purification to exclude non nitrotyrosine antibodies generated against the antigenic peptide backbone.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml.

Target

  • Relevance
    Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • Alternative names
    • NO tyrosine antibody
    • nTyr antibody

References

This product has been referenced in:
  • Zager RA  et al. Acute renal failure: determinants and characteristics of the injury-induced hyperinflammatory response. Am J Physiol Renal Physiol 291:F546-56 (2006). WB ; Mouse . Read more (PubMed: 16638912) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you again for your patience. Yes, this is correct. The result will vary based upon the sample. Any nitrosylated protein will be detected and the results will vary based on the size of the sample protein. The result you have observed is indee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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