Anti-Nitro tyrosine antibody (ab24497)

Overview

  • Product nameAnti-Nitro tyrosine antibody
    See all Nitro tyrosine primary antibodies
  • Description
    Mouse monoclonal to Nitro tyrosine
  • Specificityab24497 recognises Nitrotyrosine containing proteins.
  • Tested applicationsSuitable for: WBmore details
  • Immunogen

    Peroxynitrite treated KLH

Properties

Applications

Our Abpromise guarantee covers the use of ab24497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml.

Target

  • RelevanceNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • Alternative names
    • NO tyrosine antibody
    • nTyr antibody

References for Anti-Nitro tyrosine antibody (ab24497)

ab24497 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. A useful positive control for this antibody is protein extracted from human A431 cells (Human epithelial carcinoma cell line) or other cells pretreated with peroxynitrite for 5 min. In the following publication, they u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"