Anti-Nitro tyrosine antibody (ab27628)

Overview

  • Product name
    Anti-Nitro tyrosine antibody
    See all Nitro tyrosine primary antibodies
  • Description
    Goat polyclonal to Nitro tyrosine
  • Specificity
    Specifically binds to nitrate LDL, and other nitrated proteins.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Synthetic peptide - Nitrotyrosine (NY) modified Protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 75mM PBS, 75mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • Purification notes
    Purified by nitrated protein-SepharoseTM affinity column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: range: 1/5000 to 1/40000.
    WB: range: 1/5000 to 1/40000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
    • Alternative names
      • NO tyrosine antibody
      • nTyr antibody

    References for Anti-Nitro tyrosine antibody (ab27628)

    ab27628 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    The ab27628 Anti-Nitro tyrosine antibody is the one you are looking for:

    http://www.abcam.com/index.html?datasheet=27628 (or use the following: http://www.abcam.com/index.html?datasheet=27628).

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up