Overview

  • Product name
    Anti-Nitro tyrosine antibody
    See all Nitro tyrosine primary antibodies
  • Description
    Rabbit polyclonal to Nitro tyrosine
  • Specificity
    This antibody reacts with free and bound nitrotyrosine. There is no cross reaction with free or bound tyrosine
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WB, ELISAmore details
  • Species reactivity
    not applicable
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH: Nitrotyrosine

Properties

Applications

Our Abpromise guarantee covers the use of ab42789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 22540017
IHC-P Use at an assay dependent concentration. PubMed: 24551153
WB 1/200 - 1/2000. Predicted molecular weight: 1 kDa.
ELISA 1/5000 - 1/20000.

Target

  • Relevance
    Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • Alternative names
    • NO tyrosine antibody
    • nTyr antibody

Images


  • Developed using the ECL technique

    Predicted band size : 1 kDa
    Observed band size : 25,45,55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Gwen Lagoda by Abreview.

  • Immunohistochemical analysis of prediabetic obese rat penile tissue, staining Nitro tyrosine with ab72789.

    Samples were blocked with 10% normal goat serum, permeabilized with 0.3% Triton X-100 before being incubated with primary antibody (1/100) for 48 hours at 4°C. A biotinylated goat anti-rabbit IgG (1/400) was used as the secondary antibody and staining was detected using DAB.

References

This product has been referenced in:
  • Timpani CA  et al. Attempting to Compensate for Reduced Neuronal Nitric Oxide Synthase Protein with Nitrate Supplementation Cannot Overcome Metabolic Dysfunction but Rather Has Detrimental Effects in Dystrophin-Deficient mdx Muscle. Neurotherapeutics 14:429-446 (2017). Mouse . Read more (PubMed: 27921261) »
  • Fernandes CR  et al. High-Fat and Fat-Enriched Diets Impair the Benefits of Moderate Physical Training in the Aorta and the Heart in Rats. Front Nutr 4:21 (2017). Read more (PubMed: 28573134) »

See all 5 Publications for this product

Customer reviews and Q&As

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As we discussed, I am sending you a new vial of antibody, ab42789 as a free of charge replacement. Your new order number is *********.

The antibody is covered under our Abpromise to work in...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human Penile Tissue)
Loading amount
30 µg
Specification
Human Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-15% mini gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. Gwen Lagoda

Verified customer

Submitted Apr 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Penile Tissue)
Loading amount
20 µg
Specification
Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-15 % mini gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Gwen Lagoda

Verified customer

Submitted Feb 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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