Overview

  • Product name
    Anti-Nitro tyrosine antibody (Biotin)
    See all Nitro tyrosine primary antibodies
  • Description
    Goat polyclonal to Nitro tyrosine (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Specifically binds to nitro-LDL, and other nitrated proteins.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Chemical/ Small Molecule (Nitrotyrosine)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 75M Sodium chloride, 75mM PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    purified by nitrated protein-SepharoseTM affinity column and labeled with Biotin.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Range 1/5000 - 1/40000.
    WB: Range 1/5000 - 1/40000. Predicted molecular weight: na kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
    • Alternative names
      • NO tyrosine antibody
      • nTyr antibody

    References for Anti-Nitro tyrosine antibody (Biotin) (ab27646)

    This product has been referenced in:
    • Croca S  et al. Serum nitrated nucleosome levels in patients with systemic lupus erythematosus: a retrospective longitudinal cohort study. Arthritis Res Ther 16:R48 (2014). ELISA ; Human . Read more (PubMed: 24502558) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    The ab27628 Anti-Nitro tyrosine antibody is the one you are looking for:

    http://www.abcam.com/index.html?datasheet=27628 (or use the following: http://www.abcam.com/index.html?datasheet=27628).

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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